CERTIFIED PEDIGREE

Registered Name:  Renrock Danny Boy O'Nanjay

Reg. #: WP789833/06   Sex: Male   Date Whelped: 7/1/1997   Color: ______________________________

Notes: MCOAsp98

                                  -Ch. Deer Run Wycliff

                      -Ch. Deer Run Ivan

                      |           -Tasha Farley's First Lady

              -Ch. Deer Run Ezekiel

              | OFA MF426     |           -Ch. Grand Duke O'Fern

              |           -Le Mar's Sally

              |                       -Le Mar's Little Gem

  -Ch. Old School Primo Remo Major

  | Thyroid Normal    |                       -Ch. Deer Run Noah Massalane

  |           |           -Deer Run Florister Rufus

  |           |           |           -Ch. Nanjemoy's Etasha

  |           -Ch. Old School Ursa Major

  |                   |           -Ch. Hannibal of Makar

  |                   -Old School Trouble

  |                               -Mistress Julia of Massalane

-Nanjay's Black Star In MyCrown

| |                               -Ch. Gulph Mills Mugger

| |                   -Ch. Gulph Mills Mulcher

| |                   |           -Ch. Greenbranch Macushla

| |           -Ch. Gulph Mills Ajax III

| |           |           |           -Tamarack Donner

| |           |           -Ch. Lady Natassia Of Apple Creek

| |           |                       -Ch. Lady Pebbles Of Apple Creek

| -Nanjay's Black Jewel Of Lyndon

|             |                       -Ch. Bournewood Edwin

|             |           -Ch. Harper of Lyndon

|             |           | OFA MF-282        -Ch. Oak Ridge's Aquila

|             -Lyndon Window Chimes Lyra

|           OFA good MF577G30F|           -Peach Farm Jacob

|                     -Jacob's High Buttons

|                       OFA MF-299-T      -Jalaine Daisy

-

|                                 -Ch. Deer Run Ezekiel

|                     -Ch. Burns Hall Zackery

|                     | OFA good MF1175G42M CERF-Ch. Thorchelseaias Noel

|             -Am.Can.Ch. Matts Joshua Of Dogwood Knoll

|             |           |           -Rapscallion's Sir Winston

|             |           -Ch. Burns Hall Nicole

|             |             OFA fair MF1223F53F-Ch. Night Stalker Sarah Anne

| -Ch. Lionsire Run'n Bear Samson

| | OFA excellent MF2003E28M|                     -Ch. Deer Run Ivan

| |           |           -Ch. Deer Run Ezekiel

| |           |           | OFA MF426         -Le Mar's Sally

| |           -Lionsire Girl With The Curl

| |                   |           -Sheila's Christian Samson

| |                   -Christian Bristol Cream

| |                               -Ch. Zachary's Lady Titan

-Running Bear's Rogue Of Nanjay

  |                               -Ch. Altom's Paulas Pride

  |                   -Emphil's Montana Of Wildacres

  |                   |           -Emeline Mayflower of Columbo

  |           -General Lee Bigfoot

  |           |           |           -Sir Cassius Of Rockcliff

  |           |           -Sara Lee Wycliff

  |           |                       -Babe Ruth

  -Gabriela's Abbilene

              |                       -Ch. Willowledge Caius

              |           -Vegte's Milo Of Belly Acres

              |           |           -Ch. Camelot's Rock Of Gibraltor

              -Elaine's Daisey Magnolia

                      |           -Samuel Jaws Easter

                      -Vegte's Tooty Of Belly Acres

                                  -Princess Of Coldwater

I hereby certify that this pedigree is true and correct to the best of my knowledge and belief

Signed By: __________________________________________________  Signature: __________________________________________________

Date: ______________________________  Address: ____________________________________________________________ _______________

Cystinuria genetics – Tracy Eberheart

With the mode of transmission still incomplete, and the models that follow may

 have another factor UPenns recommendations are pretty clear. The still believe

 it to be x linked recessive with incomplete penetrance ( this would be the reason

 that you would not see as many positives out of the littler as you would expect.)

 there is another factor in play, and It is my understanding that there may be

studies coming in soon that would link the xfactor recessive with the coupling with

 the other factor ( I will not say until the studies prove it so but it may be related to

 a factor many have not considered yet) so UPENN recommendations of ..........

Now let us consider the possible outcomes of different types of matings, assuming

simple X-linked inheritance of the disease.

• Normal female to normal male – This type of mating will produce only normal offspring.

• Carrier female to normal male - When a carrier female is bred to a normal male,

the sons who receive their mother’s mutant version of the gene, also referred to as the

 mutant allele, will have the disease, since their only copy of the gene is a mutant version

. The sons who get her normal allele will be normal. Therefore, on average, 1/2 of the

 sons will be normal, and 1/2 will be affected. The daughters of the carrier female by

normal male breeding will all get a normal allele from their normal father. They will get

 either a normal allele or a mutant allele from their mother. Therefore, while all of the

 daughters will be clinically normal, approximately 1/2 of them will be carrier females.

 There are two important issues to consider here.

o The proportion 1/2 stated above is an average. Each offspring has a 50:50 chance of

getting the mutant allele from the mother. Consequently, it is possible, by chance, that a

 carrier mother will not give her mutant allele to ANY of her offspring, particularly, if she

does not produce many pups. (Think of tossing a coin for example three times and

getting three heads in a row. You wouldn’t be too surprised.) Consider a litter of six,

containing three females. If, by chance, none of the three male puppies received a mutant

 allele from their carrier dam, this carrier female would not be recognized as a carrier

 because she did not produce any affected offspring, and her daughters would not be

 recognized as being at risk of being carriers themselves.

o Since a carrier female is indistinguishable from a normal female, without any additional

 information, there is no way to know if the normal-appearing female is normal or a carrier.

In the case of cystinuria, the only way to identify a carrier female is from knowledge of her

parents or offspring. If a female had an affected parent, she is an obligate carrier, having

only the possibility of getting a mutant allele from that parent. Or, if the female produced

an affected offspring, that offspring had to get a mutant allele from the mother, making her

a carrier. This emphasizes why family information is so important.

Normal female to affected male – The normal female can only contribute normal alleles

to her offspring, while the affected male contributes his only X chromosome, which carries

the mutant allele, to all of his daughters, and contributes his Y chromosome to his sons.

Therefore, all males produced from this type of mating will be normal and ALL females

produced will be CARRIERS. Important issues here are:

o Carrier and normal females are indistinguishable, so without additional information, it

would not be possible to know if the normal-appearing female used for mating was

 normal or a carrier.

o The daughters of an affected male are obligate carriers, because all daughters get their

 father’s only X chromosome, which has a mutant allele in an affected male. This is a case

where additional information allows us to differentiate between normal and carrier females.

• Affected female to normal male – The affected female has only mutant alleles to

contribute to her offspring, and the normal male has only normal alleles. Consequently,

 ALL male puppies are AFFECTED (they get their X chromosome from their mother),

and ALL female offspring are CARRIERS. Remember that:

o Due to anatomical differences, unlike affected males, affected females are much less

likely to form stones that will cause clinical problems, and in the absence of urine testing,

are very likely to be undetected.

Carrier female to affected male – Here, the mother contributes either a mutant allele, or a

 normal allele, while the father has only a mutant allele to contribute. On average, 1/2

of the daughters will be affected and 1/2 will be carriers. Of the sons, on average, 1/2

will be affected and 1/2 will be normal. Therefore, on average, 1/2 of any litter will be affected.

The important issues are:

o As explained above, the 1/2 ratios are the average that would be seen from this type of mating.

 Within a single litter, the actual ratios will vary. Litters from this type of mating could range from

 containing no affected dogs to containing all affected dogs.

o Affected females can be produced, even when a disease shows X-linked inheritance.

• Affected female to affected male – All offspring will be affected. In the case of cystinuria,

 they may not all form stones or develop clinical signs of the disease.

o This is a good place to reiterate the additional complications present for cystinuria in particular.

 In the case of cystinuria, we know that there can be cystinuric dogs without uroliths, and that

uroliths often form for the first time after a dog has been bred. For both of these cases,

 mutant alleles that are not detected in the parents are passed from parent to offspring.

The bottom line is that the breeding of affected dogs of either gender or of carrier females will

transmit mutant alleles to offspring and may produce cystinuric animals. Complicating factors

in making breeding decisions are the facts that we do not yet have any estimate of the rate at

which dogs with excess cystine in the urine develop clinical manifestations of the disease. We

do know that dogs that have had only negative NP test results have formed cystine stones.

 What this tells us is that, presumbably due to the variability of cystine levels in the urine

, a single or even a few NP test results do not necessarily give a complete understanding

 of renal cystine transport in a dog.

If a dog is found to be positive it should not be bred period, I dont really care that you have built a

 line on it. With the mode of inheritence more than likely x linked recessive with a cofactor

 influencing it, the siblings should also be spayed/neutered, they are more than likely carrying

 the mutant gene, and the expression of that gene is dependant on the cofactor being expressed also.

 It is hard enough every day to take care of these big guys, foods, regular vet expenses ect,

why throw in a known problem in the mix??

__________________
Ranchlands Mastiffs

Registered Name: De Vine Farm's Hooch
Reg. #: WP319523/02   Sex: Male   Date Whelped: 10/6/1990   Color: dark brindle
Notes: OFAf MF1308F28M,OFEL MF50,CERF317\23&65,mVWD92%,THYRNB1-11,cystPositive
                                                              -Eng.Ch. Hollesley Medicine Man
                                          -Eng.Ch. Honeycroft Danny Boy
                                          |                       -Eng.Ch. Honeycroft Crismas Cracker
                      -Eng.Ch. Bulliff Razzermataz
                      |                           |                       -Eng.Ch. Presidents Lad of Bulliff
                      |                           -Bulliff Giselt
                      |                                               -Eng.Ch. Astelle Oven Ready of Bulliff
  -Bulliff Butchers Blade
  |                       |                                               -Ranulf of Bulliff
  |                       |                           -Khan of Bulliff
  |                       |                           |                       -Monterosa Lovely Lady
  |                       -Bulliff Vagabond
  |                                           |                       -Eng.Ch. Presidents Lad of Bulliff
  |                                           -Bulliff Tarbaby
  |                                                               -Bulliff Queen Aelfgifu
-Ch. Willowledge Brett
| |vWD Clear                                                       -Bournewood Jasper
| |                                           -Ch. Bournewood Edwin
| |                                           |                       -Bournewood Sophie
| |                       -Willowledge Woodrow
| |                       |                           |                       -Alauntes Baron
| |                       |                           -Alauntes Rose
| |                       |                                               -Alauntes Glori
| -Willowledge Pollianna
|                       |                                               -Ch. Deer Run Jericho City
|                       |                           -Ch. Deer Run Wycliff
|                       |                           |                       -Deer Run Stella
|                       -Pippin of Bearhill
|                                           |                       -Ch. Willowledge Clyde of Essex
|                                           -Ch. Reveille Arwen Evenstar
|                                             OFA MF-133-T      -Ch. Reveille By Candlelight
-

|                                                               -Ch. Deer Run Wycliff
|                                           -Ch. Deer Run Ivan
|                                           |                       -Tasha Farley's First Lady
|                       -Ch. Deer Run Ezekiel
|                       |         OFA MF426           |                       -Ch. Grand Duke O'Fern
|                       |                           -Le Mar's Sally
|                       |                                               -Le Mar's Little Gem
| -Ch. Old School Majestic Major
| |                       |                                               -Ch. Deer Run Noah Massalane
| |                       |                           -Deer Run Florister Rufus
| |                       |                           |                       -Ch. Nanjemoy's Etasha
| |                       -Ch. Old School Ursa Major
| |                                           |                       -Ch. Hannibal of Makar
| |                                           -Old School Trouble
| |                                                               -Mistress Julia of Massalane
-Old School Peersleigh Muffin
  |OFA good MF966G29F CERF                                       -Master of the Woods
  |vWD Clear Thyroid Normal                   -Ch. Odin of Dahlseide
  |                                           |                       -Dahlseide Dame Puddin-Tame
  |                       -Ch. Peersleigh Storm Sherman
  |                       |                           |                       -Ch. Willowledge Caesar III
  |                       |                           -Ch. Peersleigh Princess Carolyn
  |                       |                                               -Peersleigh Lady Lorelei
  -Ch. Storm English Legacy
                      |                                               -Ch. Harold of Shute
                      |                           -Ch. Griffith's T. Geronimo
                      |                           |                       -Ch. Sun-Belle's Muggs McGinnis
                      -Willowledge Meredith
                                          |                       -Ch. Willowledge Big City Ben
                                          -Ch. Willowledge Katrina
                                                              -Ch. Willowledge Marya ,CD
I hereby certify that this pedigree is true and correct to the best of my knowledge and belief
Signed By: __________________________________________________  Signature: __________________________________________________
Date: ______________________________  Address: ____________________________________________________________ _______________

In some threads the topic has come up that you could make a dog cystinuric by the type of food fed. This is the responses to that. Both are posted with permission:

____________________________________________________________ ___-
diet and Cystinuria

I can't believe we are going over this again. But we are.

"Cystinuria" means "cystine in urine." Period. While there may be
trace.. and I mean TRACE.. levels of cystine in the urine of dogs
without this INBORN GENETIC DEFECT, when there is cystine in the
urine beyond that, the dog has cystinuria.

Of course diet will affect LEVELS OF CYSTINE IN THE URINE OF DOGS
WITH CYSTINURIA.

But it will not make a dog who does NOT have cystinuria test as
having high levels of cystine in their urine, because their kidneys
aren't abnormal and their kidneys will do what they are supposed to
do, filter it out.

In dogs with cystinuria, however, diet makes a big difference,
because feeding certain things will cause there to be more cystine
excreted and thus, leak into the urine. That's why the researchers
want the dogs to have just eaten before urine collection, to increase
the chances that the levels will be high enough to measure. But this
only applies to dogs WHO HAVE CYSTINURIA.

You can stuff your normal dog sick on high-protein foods and you
cannot make him have high levels of cystine in his urine, because,
since he doesn't have cystinuria, his body will filter it the way
it's supposed to. You can give him PURE CYSTINE and he still won't
test as having cystinuria.

If your dog has cystine in his urine -- beyond the slightest trace --
EVER, after any kind of meal or diet, then that dog has cystinuria.

As to false positives: There are a few antibiotics that will cause,
not cystine in the urine of normal dogs, but a FALSE READING on the
nitro-prusside test. The researchers at UPenn know what those drugs
are, and if your dog is positive they will, believe me, ask you about
them. It's been so long since I sent in my dogs' info I don't
remember, but they may in fact ask you on the original submission
form.

There are no known non-genetic forms of cystinuria in any species. If
you want to lie to yourself or anyone else and say your dog, or some
dog in your dog's pedigree, didn't "really" have cystinuria despite
having cystine in his urine, or that it's caused by diet or whatever,
knock yourself out.

But it will still be a lie.

Christie Keith
Caber Feidh Scottish Deerhounds
Raising our dogs holistically since 1986
- http://www.caberfeidh.com - http://www.doggedblog.com -
http://www.caninecystinuria.com -

Yes, I was going to test him a few weeks before that but once I heard they were having a clinic I waited till then to do testing.

Just thought I mention that I had Ziggy tested a second time and he came back positive again.

Sire Lionhearted Noble Warrior	Sire CH Lionhearted Maximum Power	Sire Lionhearted Medieval Man	Sire CH Double D Cameo's Mario
			Dam Lionhearted Double D Mariah
		Dam Grizzly's Total Eclipse	Sire Avalons Tuscon Warrior
			Dam Lionhearted Blackberry's Gypsy
	Dam Lionhearted Lana Of Roswal	Sire Lionhearted King Of Midnight	Sire Avalons Tuscon Warrior
			Dam Lionhearted Blackberry's Gypsy
		Dam Kingsgate Dakota Blackfoot	Sire Greiner Hall Sherman Tank
			Dam Von Roth's Spy's Good Girl
Dam Miller's Graceful Nightstalker	Sire Lesas Duke The Mighty Man	Sire RI's Prince Sampson	Sire Tuckasee's Deejay Sampson
			Dam Deejay's Cleopatra
		Dam RI's Princess Brandy	Sire Hemmingway Beauregard
			Dam Princess Sassy Sable
	Dam Lesas Queen Lady Tabitha	Sire Cabin Creeks Ramblin Zues	Sire Grubby's Bud Light
			Dam Griffin's Lady Abigail
		Dam Almighty Athena Of The Fields	Sire Cabin Crekks Almighty Zeek
			Dam Cabin Creeks Joly Mable

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